{"id":25684,"date":"2025-08-19T10:29:31","date_gmt":"2025-08-19T08:29:31","guid":{"rendered":"https:\/\/sano.science\/?post_type=research&#038;p=25684"},"modified":"2025-08-19T10:29:42","modified_gmt":"2025-08-19T08:29:42","slug":"transcriptomic-profiling-of-brain-eating-amoeba-naegleria-fowleri-infection-in-mice-the-host-and-the-protozoa-perspectives-2","status":"publish","type":"research","link":"https:\/\/sano.science\/research\/transcriptomic-profiling-of-brain-eating-amoeba-naegleria-fowleri-infection-in-mice-the-host-and-the-protozoa-perspectives-2\/","title":{"rendered":"Transcriptomic profiling of \u201cbrain-eating amoeba\u201d\u00a0Naegleria fowleri\u00a0infection in mice: the host and the protozoa perspectives"},"content":{"rendered":"\n<h2 class=\"wp-block-heading eplus-wrapper\" id=\"h-vincent-guerlais-nina-allouch-e-ashley-moseman-alicja-w-wojciechowska-jakub-w-wojciechowski-isabel-marcelino\">Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski<a href=\"https:\/\/loop.frontiersin.org\/people\/1415601\">, <\/a>Isabel Marcelino<\/h2>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n\n\n\n<p class=\" eplus-wrapper\">New Review on Experimental Approaches to Studying Amyloid Cross-Interactions<br>A recent publication co-authored by Alicja W. Wojciechowska and Jakub W. Wojciechowski from Sano, together with an international team of researchers, offers a comprehensive overview of experimental techniques for investigating amyloid protein cross-interactions.<br>Such interactions play a central role in various pathological processes, from prion conversion to the co-occurrence of distinct systemic amyloidoses. Depending on the context, these cross-interactions can accelerate amyloid self-assembly, slow it down, or even block it entirely.<br>The review outlines the strengths and limitations of commonly used in vitro and in vivo methods, including fluorescence-based assays, high-resolution imaging (such as atomic force microscopy and cryo-electron microscopy), and spectroscopic techniques. The authors emphasise that no single method can capture the full complexity of amyloid cross-interactions. Instead, they advocate for a hybrid experimental strategy \u2014 combining techniques to validate findings, analyse fibril morphology, and confirm the formation of heterotypic fibrils.<br>By integrating diverse methodological perspectives, this work provides an essential reference for researchers aiming to better understand the mechanisms of amyloid-related diseases.<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n\n\n\n<p class=\" eplus-wrapper\"><strong>Transcriptomic profiling of \u201cbrain-eating amoeba\u201d\u00a0<em>Naegleria fowleri<\/em>\u00a0infection in mice: the host and the protozoa perspectives<\/strong><\/p>\n\n\n\n<p class=\" eplus-wrapper\"><strong>Autors<\/strong>: Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski<a href=\"https:\/\/loop.frontiersin.org\/people\/1415601\">, <\/a>Isabel Marcelino<\/p>\n\n\n\n<p class=\" eplus-wrapper\"><strong>DOI<\/strong>: <a href=\"https:\/\/doi.org\/10.3389\/fcimb.2024.1490280\">10.3389\/fcimb.2024.1490280<\/a><\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n\n\n\n\t\n    \n        \n\t\t\t<a href=\"https:\/\/www.frontiersin.org\/journals\/cellular-and-infection-microbiology\/articles\/10.3389\/fcimb.2024.1490280\/full\" target=\"_self\"  class=\"button primary \">\n\n\t\t\t\t<span>\n\t\t\t\t\tREAD HERE\n\t\t\t\t<\/span>\n\n\t\t\t<\/a>\n\n        \n    \n\n\n\n<p class=\" eplus-wrapper\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>article in journal: Frontiers in Cellular and Infection Microbiology, 2025<\/p>\n","protected":false},"featured_media":0,"template":"","research_type":[],"research_team":[113],"class_list":["post-25684","research","type-research","status-publish","hentry","research_team-structural-and-functional-genomics"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.5 (Yoast SEO v27.5) - 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Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski<a href=\"https:\/\/loop.frontiersin.org\/people\/1415601\">, <\/a>Isabel Marcelino<\/h2>\n","innerContent":["\n<h2 class=\"wp-block-heading eplus-wrapper\" id=\"h-vincent-guerlais-nina-allouch-e-ashley-moseman-alicja-w-wojciechowska-jakub-w-wojciechowski-isabel-marcelino\">Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski<a href=\"https:\/\/loop.frontiersin.org\/people\/1415601\">, <\/a>Isabel Marcelino<\/h2>\n"]},{"blockName":"core\/spacer","attrs":{"height":"30px","epAnimationGeneratedClass":"edplus_anim-3hUWbj","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n","innerContent":["\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n"]},{"blockName":"core\/paragraph","attrs":{"epAnimationGeneratedClass":"edplus_anim-fNzNzO","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<p class=\" eplus-wrapper\">New Review on Experimental Approaches to Studying Amyloid Cross-Interactions<br>A recent publication co-authored by Alicja W. Wojciechowska and Jakub W. Wojciechowski from Sano, together with an international team of researchers, offers a comprehensive overview of experimental techniques for investigating amyloid protein cross-interactions.<br>Such interactions play a central role in various pathological processes, from prion conversion to the co-occurrence of distinct systemic amyloidoses. Depending on the context, these cross-interactions can accelerate amyloid self-assembly, slow it down, or even block it entirely.<br>The review outlines the strengths and limitations of commonly used in vitro and in vivo methods, including fluorescence-based assays, high-resolution imaging (such as atomic force microscopy and cryo-electron microscopy), and spectroscopic techniques. The authors emphasise that no single method can capture the full complexity of amyloid cross-interactions. Instead, they advocate for a hybrid experimental strategy \u2014 combining techniques to validate findings, analyse fibril morphology, and confirm the formation of heterotypic fibrils.<br>By integrating diverse methodological perspectives, this work provides an essential reference for researchers aiming to better understand the mechanisms of amyloid-related diseases.<\/p>\n","innerContent":["\n<p class=\" eplus-wrapper\">New Review on Experimental Approaches to Studying Amyloid Cross-Interactions<br>A recent publication co-authored by Alicja W. Wojciechowska and Jakub W. Wojciechowski from Sano, together with an international team of researchers, offers a comprehensive overview of experimental techniques for investigating amyloid protein cross-interactions.<br>Such interactions play a central role in various pathological processes, from prion conversion to the co-occurrence of distinct systemic amyloidoses. Depending on the context, these cross-interactions can accelerate amyloid self-assembly, slow it down, or even block it entirely.<br>The review outlines the strengths and limitations of commonly used in vitro and in vivo methods, including fluorescence-based assays, high-resolution imaging (such as atomic force microscopy and cryo-electron microscopy), and spectroscopic techniques. The authors emphasise that no single method can capture the full complexity of amyloid cross-interactions. Instead, they advocate for a hybrid experimental strategy \u2014 combining techniques to validate findings, analyse fibril morphology, and confirm the formation of heterotypic fibrils.<br>By integrating diverse methodological perspectives, this work provides an essential reference for researchers aiming to better understand the mechanisms of amyloid-related diseases.<\/p>\n"]},{"blockName":"core\/spacer","attrs":{"height":"30px","epAnimationGeneratedClass":"edplus_anim-bSAcXX","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n","innerContent":["\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n"]},{"blockName":"core\/paragraph","attrs":{"epAnimationGeneratedClass":"edplus_anim-fNzNzO","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<p class=\" eplus-wrapper\"><strong>Transcriptomic profiling of \u201cbrain-eating amoeba\u201d\u00a0<em>Naegleria fowleri<\/em>\u00a0infection in mice: the host and the protozoa perspectives<\/strong><\/p>\n","innerContent":["\n<p class=\" eplus-wrapper\"><strong>Transcriptomic profiling of \u201cbrain-eating amoeba\u201d\u00a0<em>Naegleria fowleri<\/em>\u00a0infection in mice: the host and the protozoa perspectives<\/strong><\/p>\n"]},{"blockName":"core\/paragraph","attrs":{"epAnimationGeneratedClass":"edplus_anim-fNzNzO","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<p class=\" eplus-wrapper\"><strong>Autors<\/strong>: Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski<a href=\"https:\/\/loop.frontiersin.org\/people\/1415601\">, <\/a>Isabel Marcelino<\/p>\n","innerContent":["\n<p class=\" eplus-wrapper\"><strong>Autors<\/strong>: Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski<a href=\"https:\/\/loop.frontiersin.org\/people\/1415601\">, <\/a>Isabel Marcelino<\/p>\n"]},{"blockName":"core\/paragraph","attrs":{"epAnimationGeneratedClass":"edplus_anim-fNzNzO","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<p class=\" eplus-wrapper\"><strong>DOI<\/strong>: <a href=\"https:\/\/doi.org\/10.3389\/fcimb.2024.1490280\">10.3389\/fcimb.2024.1490280<\/a><\/p>\n","innerContent":["\n<p class=\" eplus-wrapper\"><strong>DOI<\/strong>: <a href=\"https:\/\/doi.org\/10.3389\/fcimb.2024.1490280\">10.3389\/fcimb.2024.1490280<\/a><\/p>\n"]},{"blockName":"core\/spacer","attrs":{"height":"30px","epAnimationGeneratedClass":"edplus_anim-bSAcXX","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n","innerContent":["\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer eplus-wrapper\"><\/div>\n"]},{"blockName":"acf\/button","attrs":{"title":"READ HERE","button_type":"link","url":"https:\/\/www.frontiersin.org\/journals\/cellular-and-infection-microbiology\/articles\/10.3389\/fcimb.2024.1490280\/full","button_style":"primary","target":"_self","button_extra_classes":""},"innerBlocks":[],"innerHTML":"","innerContent":[]},{"blockName":"core\/paragraph","attrs":{"epAnimationGeneratedClass":"edplus_anim-76gUts","epGeneratedClass":"eplus-wrapper"},"innerBlocks":[],"innerHTML":"\n<p class=\" eplus-wrapper\"><\/p>\n","innerContent":["\n<p class=\" eplus-wrapper\"><\/p>\n"]}],"meta_data":{"is_automatically_other_posts":true,"number_of_posts":"3","is_automatically_check_also_posts":true},"_links":{"self":[{"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/research\/25684","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/research"}],"about":[{"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/types\/research"}],"version-history":[{"count":3,"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/research\/25684\/revisions"}],"predecessor-version":[{"id":25687,"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/research\/25684\/revisions\/25687"}],"wp:attachment":[{"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/media?parent=25684"}],"wp:term":[{"taxonomy":"research_type","embeddable":true,"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/research_type?post=25684"},{"taxonomy":"research_team","embeddable":true,"href":"https:\/\/sano.science\/index.php\/wp-json\/wp\/v2\/research_team?post=25684"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}