New publication: comparing metagenomic sequencing platforms 

A new study co-authored by Tomasz Kościółek from Sano has been published in mSystems. The paper, “A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge,” explores how consistently different sequencing platforms capture information from the human gut microbiome.

The researchers compared 1,351 matched human gut microbiome sample pairs sequenced using the MGISEQ-2000 and Illumina NovaSeq 6000 platforms. They found a high level of agreement in taxonomic profiles between the platforms, with most bacterial species consistently detected across datasets. However, the functional profiles showed more substantial differences, highlighting the challenges of combining microbiome data generated using different sequencing approaches and pre-sequencing protocols.

The findings are particularly relevant as microbiome studies continue to grow in scale and increasingly rely on data collected across different platforms and cohorts.

Read the full article: “A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge”

A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge

Authors: Kinga Zielińska, Kateryna Pantiukh, Pawel P. Łabaj, Tomasz Kosciolek, Elin Org