Transcriptomic profiling of “brain-eating amoeba” Naegleria fowleri infection in mice: the host and the protozoa perspectives

Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski, Isabel Marcelino

New Review on Experimental Approaches to Studying Amyloid Cross-Interactions
A recent publication co-authored by Alicja W. Wojciechowska and Jakub W. Wojciechowski from Sano, together with an international team of researchers, offers a comprehensive overview of experimental techniques for investigating amyloid protein cross-interactions.
Such interactions play a central role in various pathological processes, from prion conversion to the co-occurrence of distinct systemic amyloidoses. Depending on the context, these cross-interactions can accelerate amyloid self-assembly, slow it down, or even block it entirely.
The review outlines the strengths and limitations of commonly used in vitro and in vivo methods, including fluorescence-based assays, high-resolution imaging (such as atomic force microscopy and cryo-electron microscopy), and spectroscopic techniques. The authors emphasise that no single method can capture the full complexity of amyloid cross-interactions. Instead, they advocate for a hybrid experimental strategy — combining techniques to validate findings, analyse fibril morphology, and confirm the formation of heterotypic fibrils.
By integrating diverse methodological perspectives, this work provides an essential reference for researchers aiming to better understand the mechanisms of amyloid-related diseases.

Transcriptomic profiling of “brain-eating amoeba” Naegleria fowleri infection in mice: the host and the protozoa perspectives

Autors: Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski, Isabel Marcelino

DOI: 10.3389/fcimb.2024.1490280